Journal
FOOD RESEARCH INTERNATIONAL
Volume 65, Issue -, Pages 115-120Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2014.02.001
Keywords
Silymarin; Nutraceutical; Silybin; Exclusion chromatography; Sephadex LH-20; Silychristin; Silydianin
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Funding
- Czech Science Foundation [P301/11/0662]
- Ministry of Education of the Czech Republic [LH13097, LD13041]
- Institutional Concept [RV061388971]
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Silymarin, a crude extract of the seeds of Silybum marianum L. Gaertn., is widely used as an ingredient of nutraceuticals and functional food and also as therapeutics against mild liver afflictions. Most of the beneficial effects of silymarin have traditionally been ascribed to its major component silybin. The influence of several minor constituents of the crude extract was often neglected, mostly due to their difficult isolation. In this work, we report on the development of a novel separation method using Sephadex LH-20 chromatography employing methanol as a mobile phase. Using this procedure, we were even able to separate polyphenols of identical molecular weight (isobaric), yielding ca 200 to 500 mg of the particular rare flavonolignans such as silydianin and silychristin out of a 4 g silymarin mixture within one day. (C) 2014 Elsevier Ltd. All rights reserved.
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