Journal
FOOD RESEARCH INTERNATIONAL
Volume 55, Issue -, Pages 222-228Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2013.11.018
Keywords
Monkfish (Lophius litulon); Protein hydrolysate; Pentapeptide; Antioxidant activity
Categories
Funding
- National Natural Science Foundation of China [31001109]
- State-level Spark Program [2010GA700088]
- Zhejiang Province Science Foundation [Y2110636]
- Open Foundation from Ocean Fishery Science and Technology in the Most Important Subjects of Zhejiang [20110223]
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In the current study, an efficient method had been developed to acquire the antioxidant hydrolysate of monkfish muscle protein (MPH) using trypsin by an orthogonal (L-9(3)(4)) test. Under the optimum conditions of enzymolysis time 4 h, enzyme-to-substrate ratio (E/S) 2%, enzymolysis temperature 40 degrees C and pH 8.0, the DH (Degree of hydrolysis) and hydroxyl radical scavenging activity of MPH reached 19.83 +/- 0.82% and 58.05 +/- 3.01%, respectively. By using ultrafiltration, gel filtration chromatography and reversed phase high performance liquid chromatography (RP-HPLC), three antioxidant pentapeptides were isolated from MPH, and their amino acid sequences were identified as Glu-Trp-Pro-Ala-Gln (MPH-P1), Phe-Leu-His-Arg-Pro (MPH-P2), and Leu-Met-Gly-Gln-Trp (MPH-P3) with molecular weights of 629.68 Da, 668.80 Da, and 633.77 Da, respectively. MPH-P1, MPH-P2, and MPH-P3 exhibited good scavenging activities on hydroxyl radical (EC50 0.269, 0.114 and 0.040 mg/ml), DPPH radical (EC50 2.408, 3.751, and 1399 mg/ml), and superoxide anion radical (EC50 0.624, 0.101, and 0.042 mg/ml) in a dose-dependent manner. MPH-P3 was also effective against lipid peroxidation in the model system. The antioxidant activities of MPH-P1, MPH-P2, and MPH-P3 were due to their small sizes and the presence of antioxidant and. hydrophobic amino acid residues within their sequences. The results of this study suggested that the protein hydrolysate and/or its isolated peptides might be effectively used as food additives for retarding lipid peroxidation occurring in foodstuffs. (C) 2013 Elsevier Ltd. All rights reserved.
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