Journal
FOOD RESEARCH INTERNATIONAL
Volume 50, Issue 2, Pages 574-585Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2011.03.039
Keywords
Bread component interactions; FT-IR spectroscopy; Loop and Train model; Pectins; Polyphenols; Raman spectroscopy; Secondary protein conformation
Categories
Funding
- NZ Agricultural & Marketing Development Trust
- University of Auckland
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Adding dietary fibres (DFs) and polyphenols (PPs) to bread is a convenient way to deliver the health benefits of DFs and PPs to consumers. In this study, we investigate the interactions among added pectin polysaccharides, PPs and bread components such as wheat proteins in different bread formulations. Two sets of breads with a 20% difference in the water content were prepared in the absence (Control), or presence of pectin and/or fruit PP extracts (Treated). Results show that adding pectin and PPs caused changes in the molecular conformations and polymer structure of wheat gluten and starch in the finished breads, especially in the secondary protein conformation (Amides I and II, as revealed by FTIR Spectroscopy). An increase of water content during dough formulation also affected secondary protein conformation. The Treated breads gained beta-sheets in the protein's secondary conformation at the expense of beta-turns, and contained more unordered conformations especially in the presence of berry PPs. These results were confirmed by complementary Raman spectroscopic measurements. Both hydrophobic and H bonding interactions occurred among bread components and the added PPs and pectin, which affected PP stability during breadmaking and the total extractable PP content of finished breads. Schematic Loop and Train models for breads enhanced with PPs and pectin are proposed that illustrate the possible conformational changes in wheat protein structure due to the presence of added PPs and pectin. (C) 2011 Elsevier Ltd. All rights reserved.
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