Journal
FOOD RESEARCH INTERNATIONAL
Volume 44, Issue 3, Pages 798-806Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2011.01.034
Keywords
Aqueous micelle; Antioxidant capacity; DPPH free radical; Plant extracts
Categories
Funding
- Khon Kaen University
- Thailand Research Fund [RTA5380003]
- Center for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education
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A simple and sensitive method was developed to evaluate the antioxidant capacity using a 2,2-diphenyl-1-picrylhydrazyl radical (DPPH*) incorporated in surfactants. Parameters possibly affected the DPPH* scavenging activity were investigated including the type of surfactant, concentration of surfactant, solution pH and concentration of buffer. The optimum micelle system for determining the antioxidant capacity was 2 mM CTAB in 01 M acetate buffer (pH 4.6). The IC50 values of various antioxidants were calculated and compared to those prepared in methanol. Furthermore, the rate of the reaction between DPPH* and antioxidants was investigated. The rate constants observed in the micelle system were significantly faster than those in methanol, and this allowed shorter analysis time. This method was validated through linearity, limit of detection and precision by comparing with conventional methanolic assay. The purposed method was applied to evaluate the antioxidant capacity from aqueous extracted plant samples with satisfactory results. (c) 2011 Elsevier Ltd. All rights reserved.
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