Journal
FOOD RESEARCH INTERNATIONAL
Volume 41, Issue 10, Pages 952-956Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2008.07.014
Keywords
Apis cerana; Apis mellifera; Honey major protein
Categories
Funding
- Ministry of Agriculture and Forestry, Republic of Korea
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The major proteins in honey have different molecular weights depending upon the honeybee species. To confirm the origin of major honey proteins, honey protein produced by Apis cerana or Apis mellifera were purified and analyzed by MALDI-TOF Two major proteins were identified as a major royal jelly protein 1. Although two major proteins shared primary structure, they showed different molecular weights of 56 and 59 kDa, respectively. To discriminate the honeybee species producing honey using SDS-PAGE, artificial marker proteins. 56 and 59 kDa, were produced from Escherichia coli. Two artificial marker proteins were co-electrophoresed with honey samples and the difference in molecular weight was readily distinguished by SDS-PAGE. Therefore, the measurement of major proteins in honey is a useful method to discriminate the honey that produced from different honeybee species. (C) 2008 Elsevier Ltd. All rights reserved.
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