Journal
FOOD HYDROCOLLOIDS
Volume 24, Issue 5, Pages 534-541Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2009.12.005
Keywords
Simulated gastric fluid; Emulsion; beta-Lactoglobulin; Pepsin; Ionic strength; Mucin
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Funding
- Ministry of Education, New Zealand
- Massey University
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The effects of ionic strength (0-150 mM NaCl) and the presence of mucin (0.1 wt%) on the properties of oil-in-water emulsions [20.0 wt% soy oil, stabilized by 1.0 wt% beta-lactoglobulin (beta-lg)] under simulated gastric conditions (with/without 0.32 wt% pepsin at 37 degrees C, with continuous shaking at approximately 95 rev/min for 2 h) were investigated. Changes in Z-average diameter, zeta-potential and microstructure were determined as a function of incubation time. The emulsions mixed with simulated gastric fluid (SGF) (without added pepsin) were stable at low ionic strength (>= 50 mM NaCl) but showed some aggregation at high ionic strength (>= 150 mM NaCl). Extensive droplet flocculation with some degree of coalescence was observed in emulsions with 0.32 wt% added pepsin, the flocculation being potentially accelerated in the presence of NaCl. The addition of 0.1 wt% mucin resulted in a greater extent of flocculation, possibly because of non-specific binding of mucin to the positively charged beta-lg emulsion droplets. Ionic strength and the presence of mucin had a significant influence on the rate of hydrolysis of beta-lg by pepsin. The behaviour of the emulsion in SGF was predominantly driven by electrostatic interactions, which varied as a function of digestion time, ionic strength and the presence of pepsin and mucin. (C) 2009 Elsevier Ltd. All rights reserved.
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