Occurrence and estimation of aflatoxin M1 exposure in milk in Serbia

The occurrence of AFM1 was investigated in 150 cow's, 10 goat's, 5 donkey's, 10 breasts milk and 1 infant formula samples. Analyses were done using Enzyme Linked Immunosorbent Assay (ELISA) method. AFM1 was detected in 98.7% of analyzed cow's milk samples in concentrations ranged from 0.01 to 1.2 mu g/kg. Further, even 129 (86.0%) cow's milk samples contained AFM1 in concentration greater than maximum residue levels (MRL) of 0.05 mu g/kg defined by European Union (EU) Regulation. Analysis of other types of milk showed that AFM1 was detected in 80.0% goat's, 60.0% donkey's and 60.0% of breasts milk samples. Although Serbian Regulation for MRL of AFM1 in milk has been changed and harmonized with EU Regulation in 2011, occurrence of AFM1 in milk in Serbia during 2013 resulted in Regulation changes, and MRL were changed from 0.05 to 0.5 mu g/kg. On the basis of the obtained concentrations of the AFM1 in cow's milk, collected information about average milk intake and mean body weight (bw) for different age's categories, mean ingestion of AFM1 in ng/kg per bw per day were estimated. Obtained results showed that all age's categories, especially children, are exposed with high risk related to presence of AFM1 in milk. There are only a few published data about occurrence of AFM1 in milk in Serbia and none about intake assessment for AFM1. (C) 2013 Elsevier Ltd. All rights reserved.