Journal
FOOD CONTROL
Volume 31, Issue 1, Pages 211-217Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2012.09.013
Keywords
Histamine assessment; Fish; ELISA; Scombroid poisoning; Liquid chromatography
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Funding
- Istituto Zooprofilattico Sperimentale della Puglia e Basilicata
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Histamine content was tested on 311 samples of fresh fish and fish products collected in Puglia (Italy) in the years 2009-2011. The analyses were carried out employing ELISA for screening and HPLC to confirm suspicious non-compliant samples according to Commission Regulation (EC) 1441/2007. Histamine concentration >2.5 mg kg(-1) was detected in 58% of total samples with 5% of non-compliance. Among 216 samples of fresh fish, 70% had a content of histamine less than 10 mg kg(-1), while a percentage of 6% showed histamine level higher than 100 mg kg(-1). Although for processed anchovies a contamination below 50 mg kg(-1) in 21 samples (75%) was observed, the highest percentage of non-compliant samples (14%) was found. Good quality of canned tuna and canned mackerel was demonstrated by histamine content lower than 10 mg kg(-1). The validation of both ELISA and HPLC methods and the optimization of the confirmatory method were also reported. (C) 2012 Elsevier Ltd. All rights reserved.
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