Journal
FOOD CONTROL
Volume 25, Issue 2, Pages 555-560Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2011.11.039
Keywords
Ochratoxin A; Mycotoxin biosensor; Fluorescence; Structure-switching aptamer
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Funding
- Science and Technology Bureau of Guangzhou [2008Z1-E581, 2009Ss-P030]
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A fast and reliable sensing platform has been developed for the detection of mycotoxin ochratoxin A (OTA) based on a target-induced structure-switching signaling aptamer. In the absence of target, a fluorescein-labeled OTA aptamer hybridizes to a complementary DNA strand containing a quencher moiety, bringing the fluorophore and the quencher into close proximity for highly efficient fluorescence quenching. Upon OTA addition, a conformational change in the aptamer releases the quencher-containing DNA strand, generating a strong concentration-dependent fluorescent signal. Using this technique, the entire detection and analysis process of OTA can be completed within 1 min. Under optimized assay conditions, a wide linear detection range (from 1 to 100 ng/mL) was achieved with a detection limit down to 0.8 ng/mL Additionally, the proposed assay system exhibited high selectivity for OTA against other mycotoxins (aflatoxin B-1 and zearalenone) and limited interference from the structural analog ochratoxin B. The biosensor was also applied to a non-contaminated corn material spiked with a dilution series of OTA, obtaining recoveries from 83% to 106%. Utilization of the proposed biosensor for quantitative determination of mycotoxins in food samples may provide significant improvements in quality control of food safety through a simple, rapid, and sensitive testing system for agricultural products monitoring. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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