Journal
FOOD CONTROL
Volume 21, Issue 5, Pages 633-638Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2009.08.014
Keywords
Medicinal plants; In vivo assays; Salmonella Typhimurium; Erythrocytes
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Funding
- PhD fellowship [SFRH/BD/19601/2004]
- Fundacao para a Ciencia e a Tecnologia (Portugal)
- EXTRAVIDA [13-05-04 - FDR - 00020]
- PRIME/IDEIA
- Agencia de Inovacao (Portugal)
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The bacteriophage P22/Salmonella Typhimurium system, as well as human erythrocytes have been used to assay for protection, against forced oxidation caused by hydrogen peroxide, brought about by several aqueous extracts of selected adventitious plants grown in Portugal. This study proved, for the first time, that the aforementioned bacteriophage-based system is a suitable method to assess the antioxidant activity of plant extracts; among the 12 plants tested, raspberry (Rubus idaeus), sage (Salvia sp.), savory (Satureja montana) and yarrow (Achillea millefolium) were found to effectively protect against oxidative damage caused by H2O2. Haemolysis was inhibited via pre-treatment with every plant extract tested, except heath at 0.1% (w/v). The two analytical methods produced different results - and for some plants, there was a dependence (either director inverse) of the quantitative protection effect on extract concentration, whereas for others no significant dependence was found at all. Savory yielded the most promising results, using either method. Therefore, the P22/Salmonella system can be used as a Suitable in vivo assay, and human erythrocytes as a suitable in vitro assay to confirm (or not) the antioxidant capacity of plant extracts in biological matrices. (C) 2009 Published by Elsevier Ltd.
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