Journal
FOOD CHEMISTRY
Volume 276, Issue -, Pages 333-341Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.09.164
Keywords
Fluorescent magnetic nanobeads; Immunomagnetic separation; Fluorescent lateral flow immunoassay; Escherichia coli O157:H7
Funding
- National Natural Science Foundation of China [31772066]
- Jiangxi Special Fund for Agro-scientific Research in the Collaborative Innovation [JXXTCX201703-1]
- Earmarked Fung for the Jiangxi Agriculture Research System [JXARS-03]
- State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-ZZB-201719]
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Fluorescent lateral flow immunoassay (FLFIA) based on immunomagnetic separation (IMS) has the advantage of sensitivity. However, its complex operation includes IMS, elution, incubation, and FLFIA steps. Here, we prepared a core@shell@satellite structure fluorescent magnetic nanobeads (FMNBs) and firstly introduced them into the novel method that integrated IMS with FLFIA (I-IMS-FLFIA) for the detection of Escherichia coli O157:H7. The FMNBs exhibited excellent magnetic and fluorescent properties for applications in IMS and FLFIA. However, the inner filter effect (IFE) of FMNBs may disturb the detection of I-IMS-FLFIA. Systematical studies showed that the amount of immuno-FMNBs and the concentration of monoclonal antibody can be controlled to obtain maximum photoluminescence intensity and effectively weaken or solve IFE. Under optimum conditions, this method allows for the quantified detection of 2.39x10(2) CFU/mL and qualified detection of 2.50x10(3) CFU/mL. The method is simple, safe, efficient, and sensitive for the detection of foodborne pathogens.
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