Journal
FOOD CHEMISTRY
Volume 162, Issue -, Pages 27-33Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.04.065
Keywords
Soybean; Glycinin; Soy-free diet; Polyclonal antibody; Monoclonal antibody; ELISA
Funding
- National Basic Research Program of China (973 Program) [2013CB117303]
- National Natural Science Foundation of China [31272448]
- National Twelfth Five-Year Science & Technology Pillar Program [2011BAD26B02-1]
- Chinese Universities Scientific Fund [2012QJ102, 2013QJ074, 2013QJ077]
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This study performs a sandwich ELISA for detection of trace amounts of glycinin in soybean products. We designed a soy-free mouse model to produce anti-glycinin monoclonal antibodies with high affinity and specificity. Using the monoclonal antibody as coating antibody, with the rabbit anti-glycinin polyclonal antibody as a detected antibody, the established sandwich ELISA showed high specificity for glycinin with minimum cross-reactions with other soy proteins. The practical working range of the determination was 3-200 ng/mL with detection limit of 1.63 ng/mL. The regaining of glycinin in spiked soybean samples were between 93.8% and 103.3% with relative standard deviation less than 8.3% (intra-day) and 10.5% (inter-day). The developed assay was used in analysing 469 soybean samples and five soybean products under different processing. The assay provides a specific and sensitive method for screening of glycinin and allows for further investigation into hypersensitive mechanisms to soybean proteins. (c) 2014 Elsevier Ltd. All rights reserved.
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