Nanoencapsulation of an active peptidic fraction from sea bream scales collagen

Sea bream scales were subjected to enzymatic hydrolysis with Esperase, and a peptide fraction with a molecular mass <3 kDa (F3) was isolated by ultrafiltration. F3 was encapsulated in nanoliposomes made of partially purified phosphatidylcholine (PC). Concentrations of 3.1% and 1 mg/ml for PC and F3, respectively, were established as the best entrapment protocol by response surface methodology. The liposomes entrapment efficiency and zeta potential were 74.6 +/- 0.9% and -40.8 +/- 0.67 mV, respectively. The liposome size ranged from 66.2 to 214 nm, with a mean diameter of 90.3 nm and a polydispersity index of 0.25. The antioxidant activity and ACE inhibitory activity of the encapsulated peptide fraction (L-F3) remained constant after 8 days at 4 degrees C. Encapsulation preserved the biological activities of F3, and could therefore be an alternative to improve the stability of these compounds when applied to a food product. (C) 2014 Elsevier Ltd. All rights reserved.