Journal
FOOD CHEMISTRY
Volume 149, Issue -, Pages 319-325Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.10.066
Keywords
High-performance liquid chromatography; Hollow-fibre liquid-phase microextraction; Oestrogens; Milk derivatives
Funding
- CajaCanarias
- Spanish Ministry of Economy and Competitiveness [AGL2011-24667]
Ask authors/readers for more resources
In this work, the potential of a hollow-fibre liquid-phase microextraction (LPME)-based method has been studied and validated for the extraction of a group of nine oestrogenic compounds four of them being natural (oestrio1, 17 beta-oestradiol, 17 alpha-oestradiol and oestrone), four being synthetic (17 alpha-ethynyloestradiol, diethylstilbestrol, dienestrol and hexestrol) and one metabolite (2-hydroxyoestradiol) in different dairy products (whole and skimmed natural yogurt, a probiotic yogurt-type drink and cheese). The methodology includes a prior protein precipitation with acidified acetonitrile for all samples and an additional defatting step with n-hexane for cheese, the matrix with the highest fat content. Later separation, determination and quantification were done by high-performance liquid chromatography coupled to a diode array detector and a fluorescence detector set in series. Calibration, sensitivity, precision and accuracy of the method were carried out in the selected matrices, providing good linearity, LODs in the low mu g/kg or mu g/L range, good precision and appropriate accuracy. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available