Journal
FOOD CHEMISTRY
Volume 145, Issue -, Pages 984-990Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.09.021
Keywords
Aconitase; Citrate; Citrus grandis; Granulation; Malate; Organic acid metabolism; Phosphoenolpyruvate carboxylase (PEPC); Pummelo
Funding
- earmarked fund for China Agriculture Research System
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We investigated the effects of granulation on organic acid metabolism and its relation to mineral elements in 'Guanximiyou' pummelo (Citrus grandis) juice sacs. Granulated juice sacs had decreased concentrations of citrate and isocitrate, thus lowering juice sac acidity. By contrast, malate concentration was higher in granulated juice sacs than in normal ones. The reduction in citrate concentration might be caused by increased degradation, as indicated by enhanced aconitase activity, whilst the increase in malate concentration might be caused by increased biosynthesis, as indicated by enhanced phosphoenolpyruvate carboxylase (PEPC). Real time quantitative reverse transcription PCR (qRT-PCR) analysis showed that the activities of most acid-metabolizing enzymes were regulated at the transcriptional level, whilst post-translational modifications might influence the PEPC activity. Granulation led to increased accumulation of mineral elements (especially phosphorus, magnesium, sulphur, zinc and copper) in juice sacs, which might be involved in the incidence of granulation in pummelo fruits. (C) 2013 Elsevier Ltd. All rights reserved.
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