Journal
FOOD CHEMISTRY
Volume 136, Issue 3-4, Pages 1435-1443Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.09.053
Keywords
Bioactive peptides; Whey protein isolate hydrolysates; Free radical scavenging activity (FRSA); Oxygen radical absorbance capacity (ORAC); Surface hydrophobicity; Dual functional peptides
Funding
- EH Graham Centre for Agriculture Innovation
- Charles Sturt University
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Heat pre-treated and non heat pre-treated whey protein isolate (WPI) were hydrolysed using alpha-chymotrypsin (chymotrypsin), pepsin and trypsin. The in vitro antioxidant activity, ACE-inhibition activity and surface hydrophobicities of the hydrolysates were measured in order to determine if peptides with dual functionalities were present. Dual functional peptides have both biological (e.g. antioxidant, ACE-inhibition, opioid activities) and technological (e.g. nanoemulsification abilities) functions in food systems. Heat pre-treatment marginally enhanced the hydrolysis of WPI by pepsin and trypsin but had no effect on WPI hydrolysis with chymotrypsin. With the exception of the hydrolysis by trypsin, heat pretreatment did not affect the peptide profile of the hydrolysates as analysed using size exclusion chromatography, or the antioxidant activity (P>0.05). Heat pre-treatment significantly affected the ACE-inhibition activities and the surface hydrophobicities of the hydrolysates (P<0.05), which was a function of the specificity of the hydrolysing enzyme. Extended hydrolysis (up to 24 h) had no significant effect on the DH and the molecular weight profiles (P>0.05) but in some instances caused a reduction in the antioxidant activity of WPI hydrolysates. The chymotrypsin hydrolysate showed a broad MW size range, and was followed by pepsin and then trypsin. The bioactivities of the hydrolysates generally decreased in the order; chymotrypsin > trypsin > pepsin. This study showed that by manipulating protein conformation with pre-hydrolysis heat treatment, combined with careful enzyme selection, peptides with dual functionalities can be produced from WPI for use as functional ingredients in the manufacture of functional foods. (C) 2012 Elsevier Ltd. All rights reserved.
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