Journal
FOOD CHEMISTRY
Volume 138, Issue 2-3, Pages 1588-1595Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.11.052
Keywords
beta-Galactosidase; Transglycosylation; Mutation; Lactose; Galacto-oligosaccharides
Funding
- National Natural Science Foundation of China [30970057, 31100048]
- 111 Project [111-2-06]
- 863 Program [2012AA021500]
Ask authors/readers for more resources
Galacto-oligosaccharides (GOS), an important class of functional food, are commonly produced from lactose using beta-galactosidase. In the present study, beta-galactosidase (LacS) from Sulfolobus solfataricus P2 was cloned and site-directed mutagenesis was performed to obtain two mutants, F359Q and F441Y. All of the wild-type enzyme and mutants were expressed in Escherichia coli BL21(DE3) and purified to homogeneity. The enzymatic properties and optimal condition for transglycosylation reaction of the enzymes were investigated in detail. Under their individual optimal conditions, yields of GOS could reach 50.9% for wild-type enzyme, 58.3% for F359Q and 61.7% for F441Y. In addition, the potential mechanism for this enhancement was analysed. (C) 2012 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available