Journal
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 96, Issue 6, Pages 1959-1966Publisher
WILEY-BLACKWELL
DOI: 10.1002/jsfa.7304
Keywords
aflatoxin B-1; aflatoxin B-2a; degradation; lactic acid; cytotoxicity assay
Funding
- VIT University
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BACKGROUND: A degradation study of aflatoxin B-1 (AFB(1)) was carried out using a combination of physical and chemical methods. AFB(1) was heated at 80 degrees C in the presence of acetic, citric and lactic acids for various time periods. The cytotoxicity of the degraded AFB(1) and its products were determined by MTT assay. RESULTS: The results showed that among the three organic acids lactic acid was most efficient in degrading AFB(1). Although complete degradation was not observed, up to 85% degradation of AFB1 was obtained when heated for 120 min. Degradation of AFB(1) was confirmed by the reduced toxicity on HeLa cells using MTT assay. Treatment with lactic acid resulted in the conversion of AFB(1) into two degradation products. These products were observed at lower retention factors of 0.63 and 0.38, which were identified as AFB(2) and AFB(2a,) respectively. The cytotoxicity of AFB(2a) exhibited much reduced toxicity on HeLa cells compared to that of AFB(1). CONCLUSION: The results have shown the efficiency of lactic acid in degrading AFB(1). This study suggest that lactic acid may be considered for use in the food and feed industry since it is present naturally in food and is considered safe. (C) 2015 Society of Chemical Industry
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