Journal
FOOD CHEMISTRY
Volume 138, Issue 4, Pages 2130-2139Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.10.047
Keywords
Medicinal mushrooms; Apoptosis; Cell cycle; Human hepatoma cells
Funding
- National Natural Science Foundation of China [30170775, 30671702]
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This study describes molecular mechanisms for inhibiting tumour cell proliferation using polysaccharides from medicinal mushrooms in human hepatoma cells. The results show that regarding cell cycle-related proteins, three types of polysaccharides significantly enhance the expression of p27(Kip) in HepG2 and Bel-7404 cells, while suppressing the activity of cyclin D1/CDK4 and/or cyclin E/CDK2. Considering apoptosis-related factors, the polysaccharides suppressed ART activity through the inhibition of ART phosphorylation at Thr(308) and/or Ser(473). The growth of HepG2 and Bel-7404 cells was suppressed by the up-regulation of a subunit of PI3K and phospho-PTEN, which are modulators of AKT activity. The polysaccharides also activated the mitochondria-mediated apoptosis pathway by stimulating the activation of BcI-2 family proteins to release cytochrome c and Smac and cleave caspase-9 and caspase-3 in HepG2 and Bel-7404 cells. These factors have a potent effect on cell cycle arrest in G(1) and/or S phase and induce apoptosis in HepG2 and Bel-7404 cells. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
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