Journal
FOOD CHEMISTRY
Volume 138, Issue 2-3, Pages 966-975Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.11.028
Keywords
Neisseria polysaccharea amylosucrase; Resistant starch; Structural modification; Rice starch; Barley starch
Funding
- National Research Foundation of Korea (NRF)
- Korea government (MEST) [2012R1A2A2A01047497]
- National Research Foundation of Korea [2012R1A2A2A01047497] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Structural modification of rice and barley starches with Neisseria polysaccharea amylosucrase (NpAS) was conducted, and relationship between structural characteristics and resistant starch (RS) contents of NpAS-treated starches was investigated. Pre-gelatinised rice and barley starches were treated with NpAS. NpAS-treated starches were characterised with respect to morphology, X-ray diffraction pattern, amylopectin branch-chain distribution, and RS content, and their structural characteristics were correlated to RS contents. Regardless of amylose contents of native starches, NpAS-treated (relative to native) starches possessed lower and higher proportions of shorter (DP 6-12) and intermediate (DP 13-36) amylopectin (AP) branch-chains, respectively. RS contents were higher for NpAS-treated starches relative to native starches, and maximum RS contents were obtained for NpAS-treated starches of waxy rice and barley genotypes. Amylose contents were not associated with RS contents of NpAS-treated starches. However, shorter and intermediate AP branch-chain portions were negatively and positively correlated to RS contents of NpAS-treated starches, respectively. (C) 2012 Elsevier Ltd. All rights reserved.
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