Journal
FOOD CHEMISTRY
Volume 136, Issue 3-4, Pages 1322-1329Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.09.044
Keywords
HPLC; Penicillins; Amphenicols; Gilthead seabream; Solid phase extraction
Ask authors/readers for more resources
A confirmatory high performance liquid chromatography method for the determination of six penicillin antibiotics and three amphenicol antibiotics in gilthead seabream (Sparus Aurata) tissue was developed. Ampicillin (AMP), penicillin G (PG), penicillin V (PV), oxacillin (OXA), cloxacillin (CLO), dicloxacillin (DICLO), thiamphenicol (TAP), florfenicol (FFC) and chloramphenicol (CAP) were separated on an Inertsil, C-8 (250 x 4 mm, 5 mu m) column by gradient elution with a mobile phase consisting of ammonium acetate 0.05 M and acetonitrile at 25 degrees C. Diode array detection with monitoring at 225 nm (for the determination of AMP, PG, PV, TAP and FFC), 240 nm (for OXA, CLO and DICLO) and 278 nm (for CAP) was applied. Examined antibiotics were isolated from gilthead seabream tissue by liquid-liquid extraction and further clean-up was performed by solid phase extraction using Oasis HLB (200 mg/6 mL) cartridges. The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity according to the European Union Decision 2002/657/EC. (C) 2012 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available