Journal
FOOD CHEMISTRY
Volume 141, Issue 3, Pages 2526-2532Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.05.089
Keywords
Salmonella detection; 16S rRNA; Gold nanoparticles; Lateral flow immunoassay
Funding
- Institute of Preventive Medicine, National Defense Medical Center [IPMC 101-B10]
- National Taipei University of Technology [NTUT-MMH-100-11]
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An ultrasensitive, simple, and fast lateral flow immunoassay for Salmonella detection using gold nanoparticles conjugated with a DNA probe, which is complementary to the 16S ribosomal RNA and DNA of Salmonella, has been developed. The detection limit is 5 fmol for the synthetic single-stranded DNA. For the Salmonella cultured samples, the nucleic acids from 10(7) bacteria were rapidly detected in 30 min. After silver enhancement, the detection limit was as low as 10(4) cells which is lower than 10(5) bacteria cells, the human infective dose of food-borne Salmonella. Furthermore, the probes used in this study are specific to Salmonella compared to several other Enterobacteriaceae. This approach would be a useful tool for microbial detection regarding food safety or clinical diagnosis. It is also suitable for large-scale screening in developing countries because it is low-cost, sensitive, specific and convenient. (C) 2013 Elsevier Ltd. All rights reserved.
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