Journal
FOOD CHEMISTRY
Volume 141, Issue 3, Pages 2198-2206Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.04.089
Keywords
Salvia officinalis extract; Thymus vulgaris extract; Antioxidant activity; Human hepatoma HepG2 cells; Comet assay; Antioxidant enzymes
Funding
- Scientific Grant Agency of the Ministry of Education of Slovak Republic and the Academy of Sciences (VEGA) [2/0012/12, 2/0177/11]
- Research & Development Operational Programme [ITMS: 26240120008, ITMS: 26240120030]
- ERDF
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Salvia officinalis (SO) and Thymus vulgaris (TV) are medicinal plants well known for their curative powers. However, the molecular mechanisms responsible for these abilities of sage and thyme have not been fully understood yet. In this study we investigated the composition and the quantitative estimation of plant extracts, the protective effects of plant extracts against hydrogen peroxide- and 2,3-dimethoxy-1,4-naphthoquinone-induced DNA damage, and levels of enzymatic and non-enzymatic antioxidants (superoxide dismutase, glutathione peroxidase, glutathione) in human HepG2 cells. To measure antioxidative activity of plant extracts we used three assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The results showed that the oxidant-induced DNA lesions were significantly reduced in cells pre-treated with the plant extracts studied. The observed DNA-protective activity could be explained by both elevation of GPx activity in cells pre-treated with SO and TV and antioxidant activity of SO and TV. (C) 2013 Elsevier Ltd. All rights reserved.
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