Journal
FOOD CHEMISTRY
Volume 139, Issue 1-4, Pages 274-280Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.02.007
Keywords
Quinoxaline-2-carboxylic acid; Methyl-3-quinoxaline-2-carboxylic acid; Molecularly imprinted polymer; Solid phase extraction; High-performance liquid chromatography; Animal muscle
Funding
- Ministry of Science and Technology of the People's Republic of China [2009BADB9B07, 2009BADB9B03]
- National Natural Science Foundation of China [30872126]
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A new molecularly imprinted polymer (MIP), selective for major metabolites of quinoxaline-1,4-dioxides, was prepared through bulk polymerisation using quinoxaline-2-carboxylic acid (QCA) as template, diethylaminoethylmethacrylate as functional monomer and ethylene glycol dimethacrylate as cross-linker in tetrahydrofuran. The synthesised MIP was characterised by Fourier transform infrared and adsorption experiments. MIP exhibited high affinity, fast kinetics for QCA and good selectivity for QCA and methyl-3-quinoxaline-2-carboxylic acid (MQCA). MIP obtained was used as a selective sorbent for molecularly imprinted solid phase extraction (MISPE) coupled with HPLC to detect QCA and MQCA. Under the optimal conditions, the limits of detection (S/N = 3) of porcine, chicken and fish muscles were 0.1, 0.3, 0.1 mu g/kg for QCA and 0.2, 0.3, 0.1 mu g/kg for MQCA, respectively and good recoveries were obtained in the range from 60.0 to 119.4%. These results indicated the MISPE-HPLC procedure could be successfully used for the determination QCA and MQCA in animal muscles. (C) 2013 Elsevier Ltd. All rights reserved.
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