Journal
FOOD CHEMISTRY
Volume 141, Issue 4, Pages 3820-3826Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.06.075
Keywords
Olive oil; Seeds oil; DNA extraction; PCR high resolution melting (HRM); Traceability; Adulteration
Funding
- Commission of the European Communities project [QLK1-CT-200202386]
- PETER (Promoting European Traceabilty Excellence Research) [031717]
- MENTORE (Implementation of GNSS tracking and tracing Technologies for EU regulated domains
- TRACEBACK (Integrated system for a reliable traceability of food supply chains [FP6-2005-FOOD-036300]
- Emilia-Romagna Region
- Italian Ministry of University
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Extra virgin olive oil is frequently subjected to adulterations with addition of oils obtained from plants other than olive. DNA analysis is a fast and economic tool to identify plant components in oils. Extraction and amplification of DNA by PCR was tested in olives, in milled seeds and in oils, to investigate its use in olive oil traceability. DNA was extracted from different oils made of hazelnut, maize, sunflower, peanut, sesame, soybean, rice and pumpkin. Comparing the DNA melting profiles in reference plant materials and in the oils, it was possible to identify any plant components in oils and mixtures of oils. Real-Time PCR (RT-PCR) platform has been added of the new methodology of high resolution melting (HRM), both were used to analyse olive oils mixed with different percentage of other oils. Results showed HRM a cost effective method for efficient detection of adulterations in olive oils. (C) 2013 Elsevier Ltd. All rights reserved.
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