Journal
FOOD CHEMISTRY
Volume 138, Issue 1, Pages 663-670Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.09.137
Keywords
Aurantii Immatri Pericarpium; Nobiletin; Tangeretin; Ischemia-reperfusion; Cerebral infarction
Funding
- Plant Diversity Research Center of 21st Century Frontier Research Program [PF06221-00]
- Ministry of Education, Science and Technology of Korean Government
- Regional Innovation Center Program (Research Center for Biomedical Resources of Oriental Medicine at Daegu Haany University) of the Ministry of Knowledge Economy
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Ischemic stroke is caused by brain injury due to prolonged ischemia by occlusion of cerebral arteries. In this study, we isolated active compounds from an ethanol extract of Aurantii Immatri Pericarpium (HY5356). We first showed by DNA fragmentation assay that HY5356 improved human hepatocellular carcinoma cells (HepG2) under hypoxic conditions by inhibiting apoptosis. When HY5356 was fractionated with dichloromethane (MC), ethyl acetate (EA) and n-butanol (BU), the MC fraction improved cell viability at the lowest concentration (100 mu g/ml). Intraperitoneal injection of HY5356 (200 mg/kg) or the MC fraction (200 mg/kg) to rats prior to occlusion attenuated brain injury significantly in a rat model of ischemia-reperfusion. Adopting cell viability under hypoxic conditions as an activity screening system, we isolated nobiletin and tangeretin as active compounds. The results suggest that intake of Aurantii Immatri Pericarpium containing nobiletin and tangeretin as active compounds might be beneficial for preventing ischemic stroke. (C) 2012 Elsevier Ltd. All rights reserved.
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