Journal
FOOD CHEMISTRY
Volume 140, Issue 1-2, Pages 115-123Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.02.041
Keywords
Platycodon grandiflorum; Saponins; High glucose; AMPK; Lipogenesis
Funding
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science & Tehnology (MEST) [2012-B-0012-010103]
- Priority Research Centers Program through the National Research Foundation of Korea
- Ministry of Education, Science and Technology, Republic of Korea [2009-0093815]
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saponins from the roots of Platycodon grandiflorum (Changkil saponins, CKS) have antioxidant and hepatoprotective properties. This study investigated the effects of CKS on AMP-activated protein kinase (AMPK) activation and hepatic lipogenesis in HepG2 cells. CKS suppressed high-glucose-induced lipid accumulation and inhibited high-glucose-induced fatty acid synthase (FAS) and sterol regulatory element binding protein-1c (SREBP-1c) expression in HepG2 cells. Moreover, the use of a pharmacological AMPK inhibitor revealed that AMPK is essential for the suppression of SREBP-1c expression in CKS-treated cells. Finally, the activation of calcium/calmodulin-dependent kinase kinase beta (CaMKK beta) and SIRT1 was necessary for CKS-enhanced activation of AMPK. These results indicate that CKS prevents lipid accumulation in HepG2 cells by blocking the expression of SREBP-1c and FAS through SIRT1 and CaMKK beta/AMPK activation. Using CKS to target AMPK activation may provide a promising approach for the prevention lipogenesis. (C) 2013 Elsevier Ltd. All rights reserved.
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