Pressurised liquid extraction combining LC-DAD-ESI/MS analysis as an alternative method to extract three major flavones in Citrus reticulata 'Chachi' (Guangchenpi)

Pressurised liquid extraction (PLE) of three major flavones (hesperidin, nobiletin, and tangeretin) from the peels of Citrus reticulata 'Chachi' (Guangchenpi) was investigated. These flavones were quantified and analysed by liquid chromatography-diode array detector-electrospray ionisation mass spectrometry (LC-DAD-ESI/MS). The PIE procedure was optimised, validated and compared with other conventional extraction techniques. PLE gained the best result due to the highest extraction efficiency within the shortest extraction time. The optimal conditions of PLE were employing 70% methanol as extraction solvent at a temperature of 160 degrees C and extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 20 min. MS coupling with an ESI interface in the positive ion mode was used as the detection technique. This is the first report on combining PIE with LC-DAD-ESI/MS for the extraction and quantification of flavones in Citrus reticulata 'Chachi'. (C) 2011 Elsevier Ltd. All rights reserved.