Journal
FOOD CHEMISTRY
Volume 134, Issue 4, Pages 2126-2133Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.04.011
Keywords
Rhodiola crenulata; High-speed counter-current chromotography; Semi-preparative high-performance liquid chromatography; Identification; Antioxidative activity
Funding
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20113234110001]
- National Natural Science Foundation of China [21075066]
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Six water-soluble phenylpropanoid compounds obtained from Rhodiola crenulata (R. crenulata) were fractionated by high-speed counter-current chromatography (HSCCC), and purified by semi-preparative high-performance liquid chromatography (Semi-prep HPLC). The purities of the six compounds were all above 98.0% and their structures were identified by spectroscopic methods. Among them, a new compound, 2-(4-hydroxyphenyl)-ethyl-O-beta-D-glucopyranosyl-6-O-beta-D-glucopyranoside (1), together with two known phenylpropanoids, p-hydroxyphenacyl-beta-D-glucopyranoside (3) and picein (4) were isolated from R. crenulata for the first time. Meanwhile, the contents of six isolated ingredients from the crude extract of R. crenulata had been simultaneously detected, with satisfactory results. Furthermore, the antioxidant activities of the six compounds were accessed by measuring the radical scavenging activity against 2,2-diphenyl-1-picrylhydrazy (DPPH), and four compounds exhibited potent antioxidative activity. (C) 2012 Elsevier Ltd. All rights reserved.
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