The control of Aspergillus flavus with Cinnamomum jensenianum Hand-Mazz essential oil and its potential use as a food preservative

The essential oil extracted from the bark of Cinnamomum jensenianum Hand.-Mazz was tested for antifungal activity against Aspergillus flavus. Fifty-five components accounting for 96.66% of the total oil composition were identified by GC-MS. The major components were eucalyptol (17.26%) and alpha-terpineol (12.52%). Mycelial growth and spore germination was inhibited by the oil in a dose-dependent manner. The oil also exhibited a noticeable inhibition on the dry mycelium weight and the synthesis of aflatoxin B-1 (AFB(1)) by A. flavus, completely restraining AFB(1) production at 6 mu l/ml. The possible mode of action of the oil against A. flavus is discussed based on changes in the mycelial ultrastructure. To confirm the target of the oil in the plasma membrane, studies on the inhibition of ergosterol synthesis were performed. Results show that the oil caused a considerable reduction in the ergosterol quantity. Thus, the essential oil from C. jensenianum can be used as a potential source for food preservative. (C) 2011 Elsevier Ltd. All rights reserved.