Journal
FOOD CHEMISTRY
Volume 135, Issue 3, Pages 1183-1191Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.05.044
Keywords
Enterobacter sp.; Hydrolysate; Microbial transglutaminase (MTGase); Providencia sp.; Surimi wastewater
Funding
- Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission
- Graduate School, Prince of Songkla University
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Surimi wastewater (SWW) is an industrial wastewater, released during the washing step of surimi preparation from minced fish, that causes environmental problem. In this study, SWW produced from ornate threadfin bream (Nemipterus hexodon) was hydrolysed and used to cultivate Enterobacter sp. C2361 and Providencia sp. C1112 for the production of microbial transglutaminase (MTGase, EC 2.3.2.13). The SWW was repeatedly used to wash the fish mince that gained a final protein content of 3.20% (w/v). The commercial protease, Delvolase was the most appropriate protease used to produce fish protein hydrolysate (FPH) from SWW. The FPH at 40% degree of hydrolysis was used instead of a peptone portion in the SPY medium (3.0% starch, 2.0% peptone. 0.2% yeast extract, 0.2% MgSO4, 0.2% K2HPO4 and 0.2% KH2HPO4, pH 7.0) to cultivate the tested strains at 37 degrees C, shaking speed at 150 rpm. Providencia sp. C1112 produced higher MTGase activity (1.78 +/- 0.05 U/ml) than Streptoverticillium mobaraense (1.61 +/- 0.02 U/ml) at 18 h of cultivation in FPH medium. On the other hand, the Enterobacter sp. C2361 produced lower MTGase activity (1.18 +/- 0.03 U/ml). (C) 2012 Elsevier Ltd. All rights reserved.
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