Journal
FOOD CHEMISTRY
Volume 132, Issue 1, Pages 104-111Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.10.040
Keywords
Surimi wastes; Protein hydrolysates; Antioxidant activity; HepG2 cell line
Funding
- Royal Golden Jubilee Scholarship [PhD/0023/2550]
- National Center for Genetic Engineering and Bio-technology (BIOTEC), Thailand [BT-B- 01-FT-19-5014]
- Office of the Higher Education Commission
Ask authors/readers for more resources
Antioxidant activities of protein hydrolysates from threadfin bream surimi wastes, including frame, bone and skin (FBS) and refiner discharge (RD), were investigated. FBS and RD were rich in Lys, Glu, Gly, Pro, Asp, Leu, His, Tyr and Phe. FBS was hydrolysed to a greater extent than RD regardless of proteinases tested (Virgibacillus sp. SK33 proteinase, Alcalase, pepsin and trypsin). Pepsin-hydrolysed FBS, at a 5% degree of hydrolysis (OH), showed the highest antioxidant activity based on 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulphonate) (ABTS) radical (0.455 +/- 0.054 mg Trolox equivalents/mg leucine equivalents), ferric reducing antioxidant power (FRAP) (0.221 +/- 0.005 mM Trolox equivalents) and inhibition of beta-carotene bleaching assays. FBS hydrolysates showed higher antioxidant activity based on chemical assays than their RD counterparts. However, FBS and RD hydrolysates protected HepG2 cells against tert-butyl hydroperoxide-induced oxidative damage to a similar extent. Therefore, FBS and RD hydrolysates have a potential as antioxidative neutraceutical ingredients. (C) 2011 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available