Journal
FOOD CHEMISTRY
Volume 133, Issue 2, Pages 489-496Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.01.010
Keywords
Aflatoxins; High performance liquid chromatography; Monolithic column; Foodstuffs; Liquid chromatography-tandem mass spectrometry
Funding
- Universiti Sains Malaysia (USM)
- Research Fellowship program
- [1001/PKIMIA/842071]
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A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B-1, B-2, G(1) and G(2) in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE (R) multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7 min using Chromolith (R) performance RP-18e (100-4.6 mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38-104.5% and RSDs were <4.4%. The method was applied to the determination of aflatoxins in peanut (9), rice (5) and chilli (10) samples. Liquid chromatography-tandem mass spectrometry analysis using triple quadruple analyser and operated in the multiple reaction monitoring modes on the contaminated samples was performed for confirmation. (C) 2012 Elsevier Ltd. All rights reserved.
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