Journal
FOOD CHEMISTRY
Volume 132, Issue 2, Pages 1092-1097Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.11.056
Keywords
Determination; Electrochemiluminescence; Inhibition; Phenolphthalein; Ru(bpy)(3)(2+)/DBAE system; Slimming food
Funding
- National Natural Science Foundation of China [81072336, 20805024]
- Science and Technology Department of Zhejiang Province [2010C33004, 2009C33099]
- Ningbo Science and Technology Bureau [2010A610030, 2009D10010]
- Department of Education of Zhejiang Province [Y201010064]
- Outstanding (Postgraduate) Dissertation Growth Foundation of Ningbo University
- K.C. Wong Magna Fund in Ningbo University
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An electrochemiluminescence (ECL) inhibition method combined with molecularly imprinted solid phase extraction (MISPE) was developed for quantitative determination of phenolphthalein in drug, slimming food and human plasma. Phenolphthalein could strongly inhibit the ECL signal of Ru(bpy)(3)(2+)/2-(dibutylamino)ethanol (DBAE) system that was used as the basis of the analysis. Molecularly imprinted polymers (MIPs) were synthesized as solid phase extraction (SPE) sorbents, and MISPE protocol was developed for the selective extraction and purification of phenolphthalein. Under the optimized conditions, quenched ECL intensity versus the logarithm of the concentration of phenolphthalein was in good linear relationship over a concentration range from 3.18 x 10(-10) to 1.59 x 10(-7) g/mL and the detection limit was 1.0 x 10(-10) g/mL. High affinity and selectivity of MISPE enabled the successful application of this method to determine phenolphthalein in drug, slimming food and human plasma. Further, a possible mechanism for the quenching effects of Ru(bpy)(3)(2+)/DBAE system by phenolphthalein was also proposed. (C) 2011 Elsevier Ltd. All rights reserved.
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