Journal
FOOD CHEMISTRY
Volume 134, Issue 2, Pages 1244-1249Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.03.051
Keywords
Supramolecular solvent; Microextraction; Liquid chromatography UV/VIS; spectroscopy; Carotenoids; Salmonids
Funding
- Spanish MICINN [CTQ2008-01068]
- Andalusian Government (Junta de Andalucia, Spain) [P09-FQM-5151]
- FEDER
- Andalusian CICyE
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Development of simple and rapid analytical methods for predicting supplemental feeding requirements in aquaculture is a need to reduce production costs. In this article, a supramolecular solvent (SUPRAS) made up of decanoic acid (DeA) assemblies was proposed to simplify sample treatment in the total and individual determination of carotenoids (red-pink pigments) in farmed salmonids. The analytes were quantitatively extracted in a single step that spends a few minutes using a small volume of SUPRAS (i.e. 800 mu L) and directly determined in extracts without the interference from fats or other matrix components. The methods based on the combination of microextraction with SUPRAS and photometry or HPLC-UV/VIS spectroscopy were developed for the determination of total and individual carotenoids, respectively. The applicability of the methods was demonstrated by analysing non-fortified and fortified samples of farmed Atlantic salmons and rainbow trouts. Recoveries obtained by photometry and HPLC-UV/VIS spectroscopy were within the intervals 98-104% and 94-106%, respectively. (C) 2012 Elsevier Ltd. All rights reserved.
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