Kinetics of the formation of radicals in meat during high pressure processing

The kinetics of the formation of radicals in meat by high pressure processing (HPP) has been described for the first time. A threshold for the radicals to form at 400 MPa at 25 degrees C and at 500 MPa at 5 degrees C has been found. Above this threshold, an increased formation of radicals was observed with increasing pressure (400-800 MPa), temperature (5-40 degrees C) and time (0-60 min). The volume of activation (Delta V-#) was found to have the value -17 ml mol(-1). The energy of activation (E-a) was calculated to be 25-29 kJ mol(-1) within the pressure range (500-800 MPa) indicating high independence on the temperature at high pressures whereas the reaction was strongly dependent at atmospheric pressure (E-a = 181 kJ mol(-1)). According to the effect of the processing conditions on the reaction rate, three groups of increasing order of radical formation were established: (1) 55 degrees C at 0.1 MPa, (2) 500 and 600 MPa at 25 degrees C and 65 degrees C at 0.1 MPa, and (3) 700 MPa at 25 degrees C and 75 degrees C at 0.1 MPa. The implication of the formation of radicals as initiators of lipid oxidation under HPP is discussed. (C) 2012 Elsevier Ltd. All rights reserved.