Journal
FOOD CHEMISTRY
Volume 135, Issue 2, Pages 730-737Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.05.029
Keywords
Rabdosia serra; Polysaccharide; Structural characteristics
Funding
- National Natural Science Foundation of China [31071638]
- Science and Technology Planning Project of Guangdong Province, China [2009 B011300004]
- International Foundation for Science [F/4451-2]
- Youth Innovation Promotion Association of Chinese Academy of Sciences
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Water-soluble polysaccharides of Rabdosia serra leaf and stem were fractionated by ultrafiltration and DEAE-Sepharose fast flow chromatogram to obtain water (RSLP-I and RSSP-I), 0.1 M NaCl (RSLP-II and RSSP-II) and 0.2 M NaCl (RSLP-III and RSSP-III) eluates. Their molecular weights were determined by high performance gel permeation chromatography. Monosaccharide composition analysis indicated that the water eluates comprised of rhamnose, arabinose, xylose, mannose, glucose and galactose. The ascending percentage of galactose and descending percentage of glucose in the eluates were observed with the increase of NaCl concentration. The branched RSLP-I and RSSP-I were composed mainly of -> 6)-Glcp-(1 ->, -> 6)-Galp-(1 ->, and -> 5)-Araf-(1 -> residues with the ratio of 15.0:4.2:3.8 and 5.5:6.4:3.5, respectively. However, a low level of 1,6-linked glucosyl was observed in RSLP-II, RSSP-II, RSLP-III and RSSP-III. The structural characteristics were further analysed by infrared spectrophotometry. The purified leaf and stem polysaccharides possessed moderate antioxidant capacities. (C) 2012 Elsevier Ltd. All rights reserved.
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