Journal
FOOD CHEMISTRY
Volume 133, Issue 4, Pages 1632-1635Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.03.110
Keywords
Stevioside; Steviol; Hydrolysis; On-line monitoring
Funding
- Research Foundation of the State of Sao Paulo (FAPESP)
- National Council for Scientific and Technological Development (CNPq)
- Fondecyt [1085308]
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Stevioside, a potent sweetener obtained from leaves of Stevia rebaudiana (Bertoni), is the glycone of steviol. However, despite its natural origin, there has been concern about stevioside toxicity due to hydrolysis to the carcinogenic steviol. To approve it as an additive, the FAO/WHO committee on food additives has required further information about hydrolytic stability of the steviol glycoside in acidic foods and beverages. In this study, aqueous solutions of stevioside at different pH values were monitored in real time by direct infusion ESI-MS. Owing to the high speed and sensitivity of ESI-MS monitoring, fast hydrolysis of the stevioside molecule to steviol in aqueous acidic solutions was observed, particularly in acidic juices. (C) 2011 Elsevier Ltd. All rights reserved.
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