Journal
FOOD CHEMISTRY
Volume 124, Issue 1, Pages 195-202Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.06.008
Keywords
Spinacia oleracea L; Ascophyllum nodosum; Total antioxidant capacity; Polyphenols; Flavonoids; Caenorhabditis elegans; Free radicals; Stress resistance
Funding
- Atlantic Canada Opportunities Agency (ACOA)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Nova Scotia Department of Agriculture & Marketing (NSDAF)
- Acadian Seaplants Limited
- AAFC
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There is considerable interest to enhance the nutritional quality of fresh produce especially vegetables. The effects of root treatment of spinach with commercial extracts of the brown macro alga, Ascophyllum nodosum (ANE) on antioxidant level of spinach were studied. At the concentration of 1.0 g/L, ANE treatment significantly increased the total phenolics and flavonoids content, total antioxidant activity (as measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity) and Fe2+ chelating ability in spinach leaves. The H-1 NMR and LC-MS analyses of spinach extract suggests that the increased antioxidant activity is largely associated with flavonoids. The biological effect of ANE-enhanced polyphenols was tested using the Caenorhabditis elegans nematode model. The extracts from ANE-treated spinach significantly improved the survival of the animals under oxidative stress by 50% and high temperature stress by 61% as compared to the extracts from untreated plants (0% and 38%, respectively). Taken together, the results suggest that ANE stimulate flavonoid synthesis in spinach leaf thus enhancing its nutritional quality. Furthermore, the increased flavonoid content exerts beneficial effects in C elegans against oxidative and heat stress. (C) 2010 Elsevier Ltd. All rights reserved.
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