Journal
FOOD CHEMISTRY
Volume 126, Issue 2, Pages 756-761Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.11.060
Keywords
Biogenic amine; Validation; Tuna fish; High-performance liquid chromatography
Funding
- Nutrition and Food Science Faculty of Shahid Beheshti Medical Science University of Iran
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This study compared and validated two methods of high-performance liquid chromatography: fluorescence detection (HPLC-FLD) and UV-Vis detection. Recoveries greater than 55% at all spiking levels using UV-Vis detection could not be obtained. However, derivatization with pre-column o-phthalaldehyde (OPA) was found to efficiently enhance the method sensitivity. The analytical method was validated based on the following criteria: linearity, sensitivity, accuracy, precision, repeatability, and recovery. For fluorescence detection, excellent linear correlation with R-2 = 0.9977 was observed over the range of 5.0 to 100.0 mg/L. The minimum detection limit was calculated to be 1.5 mg/kg, while the limit of quantification (LOQ) value of the validated method was determined to be 4.5 mg/kg. Good recoveries (RSD% = 1.35) were obtained at all spiking levels ranging between 0 and 200 mg/kg. The proposed method was found to be suitable, selective, and rapid for the determination of histamine. (C) 2010 Elsevier Ltd. All rights reserved.
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