Journal
FOOD CHEMISTRY
Volume 125, Issue 2, Pages 369-375Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.09.007
Keywords
Apoptosis; Guava; Caspase-3; High performance liquid chromatography coupled with photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS); Human gastric carcinoma cell; Phytochemical; SNU-16
Funding
- Koran Government (MEST) [R01-2009-000-20492-0]
- Ministry of Education, Science and Technology [2009-0094061]
- National Research Foundation of Korea [2009-0094061] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 mu g/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 mu g/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3', 4', 5' trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer. (C) 2010 Elsevier Ltd All rights reserved.
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