Journal
FOOD CHEMISTRY
Volume 129, Issue 4, Pages 1737-1743Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.06.041
Keywords
Rheum palmatum L.; Antidiarrhoeal; Tannins; Gallic acid; Catechin; LC-MS/MS
Funding
- Science and Technology Major Program [2009ZX09502-022, 2009ZX09308-005]
- Industry Research Program of Traditional Chinese Medicine [200807020]
- National Science Foundation of China [30973947]
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Though the purgative components in official rhubarb are well documented as anthraquinone glycosides and sennosides, the antidiarrhoeal effect and its chemical basis have not been reported before. In this study, liquid chromatography coupled with electrospray ionisation tandem mass spectrometry was used to investigate the main chemicals in the antidiarrhoeal fraction previously isolated from Rheum palmatum L In total 10 compounds were identified in the fraction as follows: gallic acid, galloyl glucose, di-O-galloyl-glucose, glucopyranosyl-galloyl-glucose, coumaroyl-O-galloyl-glucose, trimer of catechin, catechin gallate, catechin-glucopyranoside, carboxyl-chrysophanol-O-glucose and emodin-O-glucose. The predominant components identified in the fraction were rhubarb tannins, except for two anthraquinone glycosides of low contents. The polyphenolic analysis also showed the occurrence of abundant tannins. So, the antidiarrhoeal basis of rhubarb was primarily proposed as tannin-related compounds. (C) 2011 Elsevier Ltd. All rights reserved.
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