Journal
FOOD CHEMISTRY
Volume 124, Issue 1, Pages 50-57Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.05.102
Keywords
Furcellaria lumbricalis; Purification; Sulphated galactan; Depolymerisation; Bioactivities
Funding
- International Collaboration Program of China [2007DFA30980]
- National 863 Program of China [2007AA09Z445]
- National Natural Science Foundation of China [30870506]
- Ocean Nutrition Canada Ltd.
- Atlantic Canada Opportunity Agency (ACOA) through the PEI Food Technology Center
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The red alga, Furcellaria lumbricalis from the coast of the Prince Edward Island (PEI) in Atlantic Canada, was extracted with hot water and fractionated with 0.125 M KCl to obtain a carrageenan-like polysaccharide. The polysaccharide was further purified on ion-exchange and gel-permeation chromatography to yield a fraction (FBI) of uniform size and charge, with an average molecular weight of 428 kD. Oligosaccharides generated with acid hydrolysis of FB1 were sequenced using the electrospray ionisation collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS) technique. On the basis of chemical and spectroscopic analysis, FBI was characterised to be composed of 1,4-linked 3,6-anhydro-galactose (40%), 1,3-linked 4-sulphated-galactose (30%), 1,3-linked galactose (20%), 1,4-linked galactose (8%) and 1,4-linked 3-O-methyl-galactose (2%), which makes it be a novel sulphated galactan hybrid. The beta-secretase (BACE) inhibition and immunomodulation activities of FB1-derived oligosaccharides were evaluated in vitro. (C) 2010 Elsevier Ltd. All rights reserved.
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