Journal
FOOD CHEMISTRY
Volume 124, Issue 4, Pages 1627-1633Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.08.031
Keywords
Flavonoids; Quantification; Antioxidant effect; HPLC-MS/MS; Orostachys japonicus
Funding
- Ministry for Health, Welfare & Family Affairs, the Republic of Korea [A080164]
- Korea Health Promotion Institute [A080164] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [과06B1509] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Orostachys japonicus cultivated in the Republic of Korea was analysed for flavonoid content via HPLC coupled to MS/MS. Amongst 16 compounds that were characterised, eight fiavonoids and one alkaloid were characterised for the first time: two procyanidin dimer gallate isomers (1 and 2), epigallochatechin-3-gallate (3), two procyanidin dimer digallate isomers (4 and 9), quercetin 3-0-rhamnosy1-7-0-glucoside (6), myricetin 3-O-glucoside (10), kaempferol (16) and N-1,N-5,N-10-tri-p-(E,E,E)-coumaroylspermidine (15). The identified compounds were quantified by HPLC-UV/DAD. The antioxidant activity of the O. japonicus flavonoids was determined via 2,2-diphenyl-1-picrylhydrazyl radical (DPPH.), 2,2'-azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid) radical cation (ABTS(.+)) and nitric oxide radical (NO.) scavenging assays. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
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