Journal
FOOD CHEMISTRY
Volume 125, Issue 4, Pages 1462-1467Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.10.059
Keywords
Sudan dyes; SPE; HPLC; Food safety; Chili
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A simple rapid inexpensive and robust solid phase extraction (SPE) cleanup method coupled with high performance liquid chromatography-diode array detector (HPLC-DAD) for the determination of four Sudan dyes in chili products was developed The Interference substances were removed completely by normal SPE-Alumina N resulting in a clean and interference-free baseline Without the use of expensive LCMS/MS instrument the low levels of Sudan dyes were identified and quantified in chili foods with good precision and recovery Depending on the Sudan dyes involved the limit of determination (LOD) and limit of quantification (LOQ) were in the range of 4 1-5 8 and 13 2-19 1 mu g/kg respectively The recovery repeatability and reproducibility varied from 932% to 103% from 0 3% to 4 4% and from 0 6% to 4 9% respectively This method is suitable for the routine analysis of Sudan dyes for the common factory laboratory due to its sensitivity simplicity convenience and low-cost (C) 2010 Elsevier Ltd All rights reserved
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