Changes in the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates during a simulated gastrointestinal digestion

A two-stage in vitro digestion model system (a pepsin treatment for 2 h followed by a pancreatin treatment for 2 h, both at 37 degrees C) was used to simulate the process of human gastrointestinal (GI) digestion to determine the changes in antioxidant activities of loach peptide previously prepared by papain digestion. Results showed that the final GI digests contained 38.1% free amino acids, with short chain peptides (<500 Da) making up the rest of the biomass. Enzymatic breakdown of the GI digests increased their hydroxyl (12% increase), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) (5% increase) radical scavenging activity, the reducing power (77% increase) and the chelating ability of Cu2+ (12% increase), compared to the blank. The results showed that the digestion by gastrointestinal proteases can be used to produce antioxidant peptides, with the advantage that the peptides formed will resist physiological digestion in GI tract. (C) 2009 Elsevier Ltd. All rights reserved.