Journal
FOOD CHEMISTRY
Volume 122, Issue 4, Pages 1298-1303Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.03.086
Keywords
Nanocolloidal gold; Vegetative insecticidal protein; Bacillus thuringiensis; Nitrocellulose membrane; Immunochromatographic strip; Genetically modified crops
Funding
- Department of Biotechnology (DBT), New Delhi, India
- Council of Scientific and Industrial Research (CSIR), New Delhi
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Vegetative insecticidal protein (Vip) is now being used for transgenic expression in several crops; conferring resistance against lepidopteron pests. A rapid, single step, sensitive and specific immunochromatographic (IC) strip test for the detection of recombinant Vip-S protein in the transgenic samples was developed. Polyclonal rabbit anti-Vip-S IgG conjugated to nanocolloidal gold served as a probe to detect Vip protein in test. samples. The detection limit for the developed IC strip was 100 ng/ml(100 ppb) and on addition of gold enhancer the sensitivity increased to 1 ng/ml (1 ppb) of Vip-S protein. The assay was validated with transgenic brinjal samples. The assay time was less than 10 min, suitable for rapid on-site testing. No cross. reactivity was observed with other transgenic plant proteins employed for pest and weed management, i.e. Cry1Ac. Cry1Ab, and CP4-EPSPS. This on-site test offers rapid screening for a genetically modified crops having relatively new transgene (vip) entering the global market. (C) 2010 Elsevier Ltd. All rights reserved.
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