Journal
FOOD CHEMISTRY
Volume 120, Issue 3, Pages 825-830Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2009.11.022
Keywords
Acacia nilotica; Antioxidative assays; Natural plant products; Free radicals; Umbelliferone
Funding
- CSIR India [212241/2K7/1]
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The bark and leaves of Acacia nilotica are consumed for their promising medicinal properties in several parts of the world. The aerial portion, including flowers, is used as fodder for animals. This study aimed to isolate the functional components of A. nilotica and to check their antioxidant activities in vitro. In the fractionation of methanol extract, a fraction, AN-2, was isolated, which was identified by spectroscopic techniques, namely NMR and mass spectroscopy to be a coumarin derivative, i.e. umbelliferone. The anti-oxidative activities. including the DPPH; deoxyribose (site and non-site specific), chelating power, reducing power and lipid peroxidation assays, were studied in vitro and performed in the Dept. of Botanical and Env. Sciences, GNDU, Amritsar. It was found that the antioxidative effect of umbelliferone was dose-dependent up to 100 mu g/ml and then levelled off with no further increase in activity. This is the first report of the isolation and antioxidant potential of umbelliferone from A. nilotica. (C) 2009 Elsevier Ltd. All rights reserved.
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