Journal
FOOD CHEMISTRY
Volume 114, Issue 2, Pages 594-599Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.09.093
Keywords
Macaranga gigantea; M. pruinosa; M. tonarius; M. triloba; Antioxidant activity; Antibacterial activity; Tyrosinase inhibition
Funding
- Monash University Sunway Campus
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The methanolic fresh leaf extracts of Macaranga gigantea, Macaranga pruinosa, Macaranga tanarius and Macarango triloba were screened for their antioxidant properties (AOP), tyrosinase inhibition and antibacterial activities. Total phenolic content (TPC) 1,1 -diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, ferric-ion reducing power (FRAP), ferrous-ion chelating (FIC) and lipid peroxidation inhibition (LPI) activities were used to evaluate the AOP. Modified 3,4-dihydroxy-L-phenylalanine (L-DOPA) method was used to determine tyrosinase inhibition activity, whereas antibacterial activity was determined using the disc-diffusion technique. TPC screening of the same species from different collection sites showed no significant difference between sites. M. triloba showed the highest ascorbic acid equivalent antioxidant activity (AEAC), FRAP and LPI values. M. tanarius, which showed the lowest TPC, AEAC, FRAP and LPI activities exhibited the best FIC activity. M. pruinosa showed the best tyrosinase inhibition activity, whereas triloba showed the best antibacterial activity against Gram-positive bacteria species, with minimal inhibition dosage (MID) values as low as 10 mu g/disc. (c) 2008 Elsevier Ltd. All rights reserved.
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