Journal
FOOD CHEMISTRY
Volume 115, Issue 4, Pages 1320-1325Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2009.01.050
Keywords
Antioxidants; Phenolics; Quercus; HeLa cells
Funding
- Mexican Ministery of Education
- CONACyT-Mexico
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Water infusions of mature and fresh Quercus resinosa leaves were evaluated for antioxidant activity and genotoxic effects on HeLa cells. Native Mexicans used to drink Q. resinoso leaves tea as a refreshing beverage. The air dried leaves were pulverised and boiled in water, then their phenolic content and condensed tannins were determined. The chromatographic profile of 15 phenolic components in Quercus leaves infusions was also determined by HPLC. In vitro analysis of antioxidant capacity of leaves infusion extracts were performed by the DPPH method and the deoxyribose assay. The genotoxicity of Q. resinosa leaves extracts was evaluated on HeLa cells as well as its underlying mechanism by the single-cell electrophoresis assay (comet assay). Results show that fresh leaves infusions increase the oxidative process and other damage to DNA in transformed human cells. Fresh leaves from Q, resinosa may serve as a potential source of phenolics with anticancer activity. (C) 2009 Elsevier Ltd. All rights reserved.
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